| Themes | Hours | References | Scores |
| 1.Enzyme linked immunesorbent assay (ELISA) | Basic: 1 Supplementary: 16; 20;; 27 | 2,0 | |
| 2. Immunochromatographic assays (Lateral flow test) | Basic: 1 Supplementary: 16;; 27 | 2,0 | |
| 3. Polymerase chain reaction (PCR) | Basic: 1 Supplementary: 16; 23; 27 | 4,0 | |
| 4.Transplantation of embryos Visiting JSC “National Center for purebred breeding in Animal Husbandry” Asyl Tulik”, Koschi village, Akmola region | Basic: 1 Supplementary: 16; 24; 26; 27 | 3,0 | |
| 5. Sequencing of DNA samples using the CEQ ™ 8000 analyzer Visiting the National Center for Biotechnology branch in Stepnogorsk, Akmola region | Basic: 1 Supplementary: 16 | 4,0 | |
| Total |
List of topics for MIW
1. The new methods for the production of monoclonal antibodies and their fragments
2. Immunological methods in biotechnology of diagnostic test- systems
3 Improving technology for vaccine production.
4. Micromanipulation with embryos of domestic animals
5. State and prospects of genetic engineering in the creation of transgenic animals
6. Modern approaches in biotechnology for fodder and veterinary medicine preparations
7. Status and development prospects of Veterinary Biotechnology in Kazakhstan
8. Preparation of a literature review on the topic of dissertation.
SCHEDULE OF PASSING MIW TASKS ON DISCIPLINE
| Study subjects | Key issues | The purpose of MIW | References | Format of control | Hours | Weeks | Scores | |
| Immunological methods in biotechnology of diagnostic test- systems | Overview of ELISA in Relation to Other Disciplines; Systems in ELISA; Stages in ELISA; Titration of Reagents; Theoretical Considerations; Monoclonal Antibodies; Validation of Diagnostic Tests for Infectious Diseases; Charting Methods for Internal Quality Control; Immunochemical Techniques; immuno-PCR; ELISPOT; Aptamers | To know the modern approaches in using immunological and genetic tests in the diagnosis of zoonotic diseases. | Basic: 1 Supple-mentary: 21; 27 | Oral survey. Synopsis of MIW | 2-5 | 5.5 | ||
| Improving technology for vaccine production | Biotechnology of vaccines The main stages of vaccine production technologies. Rules and regulations of production of biopreparations. Standardization, metrology and certification of biopreparations. Validation of vaccine production. Control of vaccine companies. | Knowledge of modern approaches in technology for vaccine production. | Basic: 1 Supple-mentary: 21; 27 | Oral survey. Synopsis of MIW | 6-7 | 3.75 | ||
| Micromanipulation with embryos of domestic animals | Obtaining identical twins; Chimeric animals; Cloning of animals; Obtaining homozygous diploid offspring; Definition and sex regulation | To get acquainted with the technology of animal embryo transfer. | Basic: 1 Supple-mentary:24; 26; 27 | Oral survey. Synopsis of MIW | 2.0 | |||
| State and prospects of genetic engineering in the creation of transgenic animals | The methods of introduction of foreign DNA into the host cells; Status and prospects of obtaining and using transgenic animals; Problems in creating of transgenic animals | To get acquainted with the state and prospects of creation of transgenic animals | Basic: 1 Supple-mentary: 21; 27 | Oral survey. Synopsis of MIW | 9-10 | 3.75 | ||
| Modern approaches in biotechnology for fodder and veterinary medicine preparations | Enzymes and lipids in animal nutrition. New technologies in the production of enzymes and antibiotics for animal husbandry. | To know biotechnology for fodder and veterinary medicine preparations | Basic: 1 Supple-mentary: 25; 26; 27 | Oral survey. Synopsis of MIW | 2.0 | |||
| Status and development prospects of Veterinary Biotechnology in Kazakhstan | Determining the status and prospects of development of Veterinary Biotechnology of Kazakhstan based on a review of papers published in journals. | To prepare analysis about the status and development prospects of Veterinary Biotechnology in RK | Journal of Biotechnology: Theory and Practice (RK) | Essay on the status and prospects of Veterinary Biotechnology in RK | 2.0 | |||
| Preparation of a literature review on the topic of dissertation. | The content of a literature review is determined on the basis of dissertation’s purpose and objectives. | Papers on the topic of disser-tation. | Database of Publishing houses in the world | Literature review on the topic of disser-tation | 13-15 | 3,5 | ||
REFERENCE
8.1Basic literature:
The Basic literature of the discipline are articles and reviews published in scientific journals and proceedings of the symposiums (conferences) on current issues of Biotechnology in Veterinary Medicine and Animal Husbandry from the databases of Elsevier, Springer Science, Thomson Reuters, Pub Med and other publishing houses.
8.2 Supplementary literature:
1. Douglas P. Systematic Literature Searching and the Bibliographic Database Haystack www.ejbrm.com/issue/download.html?idArticle=195.
2. Arms W.Y., Aya S., Dmitriev P., Kot B., Mitchell R. and Walle L. (2006) “A research library based on the historical collections of the internet archive”, D-LibMagazine, vol. 12, no. 2, Feb.
3. Bachmann L.M., Coray R., Estermann P. and ter Riet G. (2002) “Identifying Diagnostic Studies in MEDLINE: Reducing the Number Needed to Read“, Journal of the American MedicalInformatics Association;9: 653-658.
4. Bradshaw J.R. (2005) Methodologies for socially useful systematic reviews in social policy, Economic and Social Research Council Research report, Swindon, UK.
5. Crawford J. (2006), “The use of electronic services and information literacy: a Glasgow Caledonian University study”, Journal of Librarianship and Information Science, vol. 38, no. 1, pp. 33-44.
6. https://www.internationalstudent.com/essay_writing/essay_tips.
7. https://www.oxford-royale.co.uk/articles/tips-techniques-essay-writer.html.
8. https://www.fastweb.com/student-life/articles/essay-tips-7-tips-on-writing-an-effective-essay.
9. Kreuzep H. and A.Massey. Molecular Biology and Biotechnology.- Washington.-ASM PRESS, 2008.-485 p.
10. Clark D.P.and N.J.Pazdernik. Cell Biotechnology. – Elsevier Inc., 2012.- 750 p.
11. Chauhan A.K.and A.A.Varma. Molecular Biotechnology. –I.K.International Publishity House Pvt.Ltd., 2009.- 1337 p.
12. Kun L.Y. Microbial Biotechnology.- Word Scientific Publishing, 2006.- 794 p.
13. Crommelin D., R.Sindelar and B.Meibohn. Pharmaceutical Biotechnology. – N.Y. London: Informa healthcare,___.-490 p.
14. Croves M.. Pharmaceutical Biotechnology. – Taylor &Francis Group, 2006.- 411 p.
15. Shetty K., G.Paliiyath, A.Pometto and R.Levin. Food Biotechnology.- Taylor &Francis Group, 2006.- 1982 p.
16. Bulashev A.K. Educational-methodical complex (EMC) on discipline "Recent developments of biotechnology in veterinary medicine and animal husbandry".- Publishing house of Seifullin KazATU: Astana, 2012.-115 Р.
17 Алмагамбетов К.Х. Биотехнология микроорганизмов.- Астана: Изд-во ЕНУ им. Гумилева, 2008.- 244 с.
18. Алмагамбетов К.Х. Медицинская биотехнология. – Астана: Изд-во ЕНУ им. Гумилева, 2009.- 236 с.
19. Булашев А.К. Моноклональные антитела в диагностике бруцеллеза. Акмола: Изд-во Акмолинского аграрного университета, 1995.-214 с.
20. Булашев А.К. Иммуноферментный анализ в диагностике бруцеллеза и туберкулеза. Астана: Изд-во Казахского аграрного университета им.С.Сейфулина, 2003.- 52 с.
21. Булашев А.К., Кухарь Е.В. Ветеринарная биотехнология.- Астана: Изд-во КазАТУ им.С.Сейфуллина, 2009.- 222 с.
22. Васильев Д.А. и соавт. (Электронный ресурс).- Лекций по курсу:Биотехнология.- Ульяновск, 2005.-188 с.
23. Глик Б., Пастернак Дж. Молекулярная биотехнология. Принципы и применение. Пер. с англ. М.: Мир, 2002.-583 с.
24. Завертяев Б.П. Биотехнология в воспроизводстве и селекции крупного рогатого скота. Л.: Агропромиздат, Ленинградское отделение, 1989.-255 с.
25. Основы биотехнологии /Т.А.Егорова, С.М.Клунова, Е.А.Живухина.- М:Издательсктй центр «Академия», 2003.-208 с.
26. Сельскохозяйственная биотехнология /В.С.Шевелуха, Е.А.Калашникова, Е.С.Воронин и др.; Под ред. В.С.Шевелухи – 2-е изд., перераб. И доп.- М:Высш.шк., 2003.-469 с.
27. Bulashev A.K. Slides on the topics of the subject.
COURSE POLICY
1. Masters must come to classes pre-prepared for the forthcoming theme of the lecture with an essay on the topic.
2. The lecture takes place in the form of discussion.
3. Masters are awarded scores based on the quality of the essay and activity during the discussion of the lecture questions.
KNOWLEDGE ASSESSMENT
Assessment criteria:
• knowledge of the latest achievements of world science as well as scientists of S.Seifullin Kazakh Agro-Technical University on improvement methods of diagnosis, treatment and prevention of infectious and parasitic diseases that cause economic and social damage to the country;
• mastering modern approaches to the creation of strains of prokaryotic and eukaryotic microorganisms and mammals - the producers of biologically active substances;
• acquaintance with state, problems and tendencies of development of the cellular and genetic engineering in veterinary medicine and animal husbandry
• knowledge of issues related to biosafety in modern Biotechnology
•the ability to solve problems by applying acquired knowledge
GRADING SYSTEM
The policy of assessment is based on a 100-scores system and provides the following distribution of points: the current and intermediate control is assigned a total of 60 scores, the final control - 40 scores.