Top Score: 369 for gi|2781338, Chain A, 1:2 Complex Of Human Growth Hormone With Its Soluble Binding Protein
| NCBInr | Decoy | |
| Protein hits above identity threshold | ||
| Highest scoring protein hit |
Mascot Score Histogram
Protein score is -10*Log(P), where P is the probability that the observed match is a random event.
Protein scores greater than 88 are significant (p<0.05).
Приложение 3. Хроматограммы с УФ и масс-детекторами стандартного (эталонного) образца соматропина.
Хроматограмма с УФ детектором.
Масспектр (положительный) пика с временем удерживания 12,0
Результаты деконволюции.
Component Molecular Average Molecule Absolute Relative
Mass Mass Abundance Abundance
A 22121.2 22121.2 [M + H]+ 183116 98,12
B 22116.1 22116.1 [M + H]+ 120577 65.85
C 24235.3 24235.3 [M + H]+ 15680 8.56
D 11065.6 11065.6 [M + H]+ 30998 16.93
E 22137.4 22137.4 [M + H]+ 29489 16.10
F 27391.2 27391.2 [M + H]+ 14461 7.90
G 17190.4 17190.4 [M + H]+ 13520 7.38
H 32420.9 32420.9 [M + H]+ 14897 8.14
I 22129.3 22129.3 [M + H]+ 21880 11.95
J 19107.6 19107.6 [M + H]+ 8735 4.77
Component A Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1107.0 20+ 22120.8 1107.0
1165.3 19+ 22121.8 1165.3
1230.0 18+ 22122.0 1230.0
1302.2 17+ 22120.5 1302.2
Molecular Mass ([M + H]+): 22121.2 Std. Deviation: 0.439267
Average Mass ([M + H]+): 22121.2
Component B Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1383.3 16+ 22117.1 1383.3
1475.3 15+ 22114.7 1475.3
1580.6 14+ 22115.7 1580.7
1702.0 13+ 22114.3 1702.1
Molecular Mass ([M + H]+): 22116.1 Std. Deviation: 0.812107
Average Mass ([M + H]+): 22116.1
Component C Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
970.4 25+ 24235.2 970.4
1010.8 24+ 24236.8 1010.9
1054.7 23+ 24235.2 1054.7
Molecular Mass ([M + H]+): 24235.3 Std. Deviation: 0.270427
Average Mass ([M + H]+): 24235.3
Component D Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1107.5 10+ 11065.5 1107.5
1230.4 9+ 11065.8 1230.4
1384.1 8+ 11065.6 1384.1
Molecular Mass ([M + H]+): 11065.6 Std. Deviation: 0.13924
Average Mass ([M + H]+): 11065.6
Component E Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1166.1 19+ 22137.3 1166.1
1230.8 18+ 22137.0 1230.8
1303.2 17+ 22138.0 1303.2
Molecular Mass ([M + H]+): 22137.4 Std. Deviation: 0.400863
Average Mass ([M + H]+): 22137.4
Component F Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
979.2 28+ 27390.1 979.2
1015.5 27+ 27393.2 1015.5
1054.5 26+ 27391.1 1054.5
Molecular Mass ([M + H]+): 27391.2 Std. Deviation: 0.602966
Average Mass ([M + H]+): 27391.2
Component G Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1075.3 16+ 17190.3 1075.3
1146.9 15+ 17189.9 1147.0
1228.8 14+ 17190.4 1228.8
Molecular Mass ([M + H]+): 17190.4 Std. Deviation: 0.28961
Average Mass ([M + H]+): 17190.4
Component H Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
754.9 43+ 32419.2 754.9
773.0 42+ 32426.7 773.1
791.7 41+ 32419.7 791.7
Molecular Mass ([M + H]+): 32420.9 Std. Deviation: 0.783483
Average Mass ([M + H]+): 32420.9
Component I Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1302.6 17+ 22128.5 1302.7
1384.1 16+ 22130.1 1384.1
1476.2 15+ 22128.5 1476.2
Molecular Mass ([M + H]+): 22129.3 Std. Deviation: 0.632705
Average Mass ([M + H]+): 22129.3
Component J Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
956.3 20+ 19106.9 956.3
1006.6 19+ 19107.7 1006.6
1062.4 18+ 19106.7 1062.5
Molecular Mass ([M + H]+): 19107.6 Std. Deviation: 0.625527
Average Mass ([M + H]+): 19107.6
Приложение 4. Хроматограммы с УФ и масс-детекторами исследуемого образца.
Хроматограмма с УФ детектором.
Масспектр (положительный) пика с временем удерживания 11,9 мин.
Результаты деконволюции.
Component Molecular Average Molecule Absolute Relative
Mass Mass Abundance Abundance
A 22119.5 22119.5 [M + H]+ 611424 100.00
B 22108.2 22108.2 [M + H]+ 124121 20.30
C 22122.9 22122.9 [M + H]+ 238081 38.94
D 22115.0 22115.0 [M + H]+ 117679 19.25
E 21069.5 21069.5 [M + H]+ 136770 22.37
F 49817.7 49817.7 [M + H]+ 33517 5.48
G 32422.1 32422.1 [M + H]+ 49934 8.17
H 23126.2 23126.2 [M + H]+ 28263 4.62
I 14088.8 14088.8 [M + H]+ 38455 6.29
J 47816.9 47816.9 [M + H]+ 22588 3.69
Component A Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1106.8 20+ 22117.8 1106.9
1165.3 19+ 22122.6 1165.3
1229.8 18+ 22118.4 1229.8
1302.1 17+ 22119.4 1302.1
1383.4 16+ 22118.8 1383.4
Molecular Mass ([M + H]+): 22119.5 Std. Deviation: 0.640608
Average Mass ([M + H]+): 22119.5
Component B Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
922.1 24+ 22106.8 922.1
962.1 23+ 22107.1 962.2
1006.0 22+ 22111.2 1006.0
1053.8 21+ 22109.1 1053.8
1106.3 20+ 22107.5 1106.4
1164.5 19+ 22107.5 1164.5
Molecular Mass ([M + H]+): 22108.2 Std. Deviation: 0.863575
Average Mass ([M + H]+): 22108.2
Component C Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
962.8 23+ 22123.2 962.8
1006.7 22+ 22125.3 1006.6
1054.4 21+ 22122.4 1054.4
1107.1 20+ 22122.9 1107.1
Molecular Mass ([M + H]+): 22122.9 Std. Deviation: 0.335916
Average Mass ([M + H]+): 22122.9
Component D Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1475.3 15+ 22115.6 1475.3
1580.5 14+ 22113.7 1580.6
1702.1 13+ 22115.5 1702.1
Molecular Mass ([M + H]+): 22115.0 Std. Deviation: 0.856926
Average Mass ([M + H]+): 22115.0
Component E Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
958.8 22+ 21071.8 958.8
1004.3 21+ 21070.9 1004.3
1054.4 20+ 21069.0 1054.4
Molecular Mass ([M + H]+): 21069.5 Std. Deviation: 0.411921
Average Mass ([M + H]+): 21069.5
Component F Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1108.0 45+ 49817.7 1108.0
1133.3 44+ 49820.3 1133.3
1159.5 43+ 49817.2 1159.5
Molecular Mass ([M + H]+): 49817.7 Std. Deviation: 0.390706
Average Mass ([M + H]+): 49817.7
Component G Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
791.7 41+ 32420.0 791.7
811.4 40+ 32417.9 811.5
832.4 39+ 32425.8 832.4
Molecular Mass ([M + H]+): 32422.1 Std. Deviation: 0.884767
Average Mass ([M + H]+): 32422.1
Component H Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
1006.5 23+ 23126.2 1006.5
1052.1 22+ 23124.9 1052.2
1102.2 21+ 23127.0 1102.3
Molecular Mass ([M + H]+): 23126.2 Std. Deviation: 0.797202
Average Mass ([M + H]+): 23126.2
Component I Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
940.2 15+ 14088.5 940.2
1007.3 14+ 14088.8 1007.3
1084.7 13+ 14089.0 1084.7
Molecular Mass ([M + H]+): 14088.8 Std. Deviation: 0.202897
Average Mass ([M + H]+): 14088.8
Component J Detail
Actual Charge Isotopic Predicted
Peak Mass ([M + H]+) Peak
870.4 55+ 47816.4 870.4
886.5 54+ 47817.1 886.5
903.2 53+ 47815.4 903.2
Molecular Mass ([M + H]+): 47816.9 Std. Deviation: 0.438304
Average Mass ([M + H]+): 47816.9